| Target Molecule | Glucagon Hydrochloride (Glucagon HCl) |
| Synonyms | Glucagon (1-29) (human) hydrochloride, GlucaGen, pancreatic glucagon HCl |
| CAS Number | 11140-85-5 (hydrochloride salt); 16941-32-5 (free base peptide) |
| Sequence (3-Letter) | H-His-Ser-Gln-Gly-Thr-Phe-Thr-Ser-Asp-Tyr-Ser-Lys-Tyr-Leu-Asp-Ser-Arg-Arg-Ala-Gln-Asp-Phe-Val-Gln-Trp-Leu-Met-Asn-Thr-OH |
| Sequence (1-Letter) | HSQGTFTSDYSKYLDSRRAQDFVQWLMNT |
| Molecular Formula | C₁₅₃H₂₂₆ClN₄₃O₄₉S (hydrochloride salt) |
| Molecular Weight | 3519.2 (hydrochloride salt); 3482.75 (free base) |
| Service Category | Custom Peptide Synthesis / CDMO Manufacturing |
| Synthesis Purity | Customizable (e.g., ≥98% via HPLC) |
| Appearance | White to off-white lyophilized powder |
| Counter Ion | Chloride (hydrochloride salt), acetate, or custom options |
| Synthesis Technology | Solid-Phase Peptide Synthesis (SPPS) for Aggregation-Prone Linear Sequences |
| Modification Options | Oxidation control, stable isotope labeling (13C, 15N), structural analogue synthesis, oxidized variant impurity standards |
| Available Scale | Milligram (R&D) to Kilogram (Commercial CDMO) |
| Analytical Data | Comprehensive COA, HPLC, MS, and salt form verification provided per batch |
| Usage | Raw material synthesized strictly for laboratory research, analytical development, and industrial formulation. Not a finished medicine. |
Glucagon Hydrochloride (CAS 11140-85-5) is the hydrochloride salt form of the 29-amino acid human peptide hormone glucagon (HSQGTFTSDYSKYLDSRRAQDFVQWLMNT). We target this sequence to supply high-purity reference materials and raw materials for metabolic receptor and diabetes research.
Assembling the 29-residue linear chain presents severe aggregation risks due to strong beta-sheet propensity. Our peptide synthesis platform applies chaotropic solvent systems, low-loading resins, and elevated-temperature coupling protocols to disrupt intermolecular hydrogen bonding and ensure complete chain elongation.
Yes. We provide specialized peptide modification services for glucagon research. Capabilities include site-specific stable isotope labeling (13C, 15N) for quantitative LC-MS/MS, fluorescent tagging, and the synthesis of specific oxidized variants (such as Met27 sulfoxide) as stability-indicating impurity standards.
Our manufacturing infrastructure scales production from milligram-level analytical batches to multigram and kilogram commercial quantities. We optimize low-pH preparative HPLC purification and precise counter-ion conversion to yield the highly soluble hydrochloride salt form consistently.
We operate as an integrated technical partner providing complete peptide CDMO solutions. Our team manages sequence optimization, aggregation suppression, impurity profiling, analytical method validation, and regulatory documentation to accelerate complex preclinical pipelines.
Glucagon Hydrochloride (CAS 11140-85-5) is the hydrochloride salt form of glucagon, a 29-amino-acid linear peptide hormone responsible for counter-regulating insulin. Defined by the sequence HSQGTFTSDYSKYLDSRRAQDFVQWLMNT, it acts as a potent glucagon receptor (GCGR) agonist. Synpeptide provides comprehensive custom synthesis solutions for this molecule, showcasing our technical capabilities in managing severe aggregation tendencies, oxidation suppression, and CDMO manufacturing.
Constructing the 29-residue Glucagon sequence requires strict control over synthetic cycles to prevent severe on-resin aggregation and deletion sequences. Our custom peptide synthesis platform resolves these hurdles using optimized Solid-Phase Peptide Synthesis (SPPS) protocols on low-loading resins. By deploying chaotropic agents, temperature control, and specific cleavage scavengers to protect Met27 and Trp25 from oxidation, we deliver raw materials with rigorous purity specifications (typically ≥98% via HPLC).
The physical properties of Glucagon demand specialized analytical validation, as the free peptide is prone to fibrillization in neutral aqueous solutions. Through our peptide modification services, we navigate these solubility constraints by optimizing pH-dependent handling procedures. Furthermore, we synthesize specific oxidized variants and structural analogues to serve as vital reference standards for stability-indicating analytical assays.
Transitioning an aggregation-prone peptide from laboratory scale to commercial volume demands robust downstream process engineering. We execute large-scale peptide synthesis for Glucagon Hydrochloride by utilizing low-pH gradient HPLC purification to prevent fibrillization. We offer precise counter-ion control, converting the peptide into the widely utilized hydrochloride salt form across all production volumes.
We deliver comprehensive technical support as a fully established peptide CDMO. Our development specialists assist pharmaceutical partners with process optimization, stability testing, impurity identification, and analytical method validation. Every batch is released with complete documentation—including COA, HPLC purity traces, and exact mass confirmation—ensuring regulatory alignment for downstream therapeutic programs.
